Translational_Unit

Part:BBa_K1500001:Design

Designed by: Kevin Yang   Group: iGEM14_UChicago   (2014-10-17)


ParoF-mCherry backbone


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In order to insert mutator genes downstream of the mCherry-LVA, we would cut open the two downstream restriction sites, with corresponding cuts on the mutator genes, before ligating the insert and this plasmid together.


Source

mCherry-LVA (BBa_J06505) was used and came from the registry plasmid. ParoF was cloned from wild-type DH5-alpha E. coli genomic sequence. RBS.3 (BBa_B0032) sequence originally came from the registry and was designed directly into primer overhangs for mCherry-LVA.

References